About

Dr. Loy is a professor, veterinary diagnostic microbiologist and faculty supervisor for the bacteriology and molecular diagnostic laboratories in the Nebraska Veterinary Diagnostic Center. His area of focus includes the application of bacterial genomics, MALDI-TOF mass spectrometry, infrared spectrometry (FT-IR), and PCR to veterinary pathogen detection and characterization. Primarily these efforts are focused on the opportunistic pathogens associated with bovine respiratory and ocular diseases, as well as antimicrobial resistance. He is an instructor in both the Veterinary Science undergraduate curriculum and the Professional Program in Veterinary Medicine.

Education

• Diplomate American College of Veterinary Microbiologists (Bacteriology/Mycology, Virology and Immunology specialties), 2012
• Ph.D. in Veterinary Microbiology, Iowa State University, 2011
• D.V.M. in Food Animal Medicine, Iowa State University, 2009
• B.S. in Animal Science, Iowa State University, 2005

Professional Responsibilities and Activities

• Teaching
  Dr. Loy is lead instructor for VMED 688 Public Health and a co-instructor for BIOS/VBMS 441/841 Pathogenic Microbiology.
• Research
  Dr. Loy's research interests include development and validation of novel molecular and proteomic diagnostic methods, pathogenesis and ecology of opportunistic bovine bacterial respiratory and ocular pathogens and enteric diseases of swine.
• Outreach
  As a diagnostic microbiologist, Dr. Loy is involved in assisting veterinarians and their clients, livestock producers and other stakeholders in the diagnosis, detection, and prevention of infectious disease. The VDC bacteriology laboratory also provides analysis and data to help inform veterinarian's decisions on prudent treatment of pathogens through performing antimicrobial susceptibility testing.

Publications

Crosby W.B., Pinnell L.J., Richeson J.T., Wolfe C., Loy J.D., Gow, S.P., Seo K.S., Capik S.F., Woolums A.R., and Morley P. S. (2022) Does swab type matter? Comparing methods for Mannheimia haemolytica recovery and upper respiratory microbiome characterization in feedlot cattle. Animal Microbiome. Vol 13 (4) 49. DOI: 10.1186/s42523-022-00197-6

Vander Ley, B.W, Loy J.D. and Woolums, A.R. (2022) Using Mental Models to Improve Understanding of Cattle Diseases. Veterinary Clinics of North America: Food Animal Practice. Vol 38 (2) 219-277. DOI: 10.1016/j.cvfa.2022.02.004

Hille M.M., Spangler M.L., Clawson M.L., Heath K.D., Vu H.L.X., Rogers R., and Loy J.D. (2022). A 5-year randomized control trial to assess the efficacy and humoral response of a commercial and autogenous vaccine for the prevention of infectious bovine keratoconjunctivitis. Vaccines. Vol. 10 (6) 916. DOI: 10.3390/vaccines10060916

Carter, H.F., Wills R.W., Scott M.A., Thompson A.C., Singer R.S., Loy J.D., Karisch B.B., Epperson W.B., and Woolums A.R. (2022) Diversity of antimicrobial resistance phenotypes and genotypes of Mannheimia haemolytica isolates from bovine nasopharyngeal swabs. Frontiers in Veterinary Science. Vol 9. 83389. DOI: 10.3389/fvets.2022.883389

Waltenburg, M.A., Shugart M.A., Loy J.D. Tewari D., Zhang S. Cole S. Ceric C. Walters M.S, and Nichols M. (2022) A survey of current activities and technologies used to detect carbapenem resistance in bacteria isolated from companion animals at veterinary diagnostic laboratories—United States, 2020. Journal of Clinical Microbiology. Journal of Clinical Microbiology. Vol 60 (3) e02154-21. DOI: 10.1128/jcm.02154-21

Sutton, K.M., Eaton C.W., Borza T., Harris S.P., Burkey T.E., Mote B.E., Loy J.D., and Ciobanu D.C. (2022) Genetic Diversity and Enhanced Detection of Atypical Porcine Pestivirus Infections. Journal of Animal Science. Vol 100 (1); skab360. DOI: 10.1093/jas/skab360

Loy, J. D., Maier G., Hille M. and Clawson M. (2021). Component causes of IBK - The Role of Moraxella spp. in the epidemiology of Infectious Bovine Keratoconjunctivitis. Veterinary Clinics: Food Animal Practice. Vol 37(2) 279-293. DOI: 10.1016/j.cvfa.2021.03.004

Hille M., Dickey A., Clawson M.L. and Loy J.D. MALDI-TOF MS biomarker detection models to distinguish RTX toxin phenotypes of Moraxella bovoculi strains are enhanced Using calcium chloride supplemented agar. (2021). Frontiers in Cellular and Infection Microbiology. 11:202. DOI:10.3389/fcimb.2021.632647

Loy, J. D. Maier G., and Clothier K. Component causes of IBK - The Role of Non-Moraxella organisms in the epidemiology of Infectious Bovine Keratoconjunctivitis. (2021). Veterinary Clinics: Food Animal Practice. Vol 37(2) 295-308. DOI:10.1016/j.cvfa.2021.03.005

Dutta E., Loy J.D., Deal C.A., Wynn E., Clawson M.L., Clarke J. and Wang B. Development of a multiplex real-time PCR assay for detection of major antimicrobial resistant bacterial pathogens associated with bovine respiratory disease complex (2021). Pathogens. 10:64. DOI: 10.3390/pathogens10010064

Bartenslager A.C., Althuge N.D., Loy J.D., Hille M.W., Spangler M.L., and Fernando S.C. (2021) Longitudinal assessment of the bovine ocular microbiome. Animal Microbiome 3:16. DOI:10.1186/s42523-021-00079-3

Loy J.D. Development and Application of Molecular Diagnostics and Proteomics to Bovine Respiratory Disease (BRD). (2020) Animal Health Research Reviews Vol 21(2) pp. 164-167. DOI:10.1017/S1466252320000092

Loy J. D., Leger L., Workman A., Bulut E., and Wang B. Application and comparison of a multiplex real-time hydrolysis probe polymerase chain reaction assay using two instrument platforms for detection of bacterial pathogens associated with bovine respiratory disease in nasal swab, nasopharyngeal swab, and lung tissue matrices. (2018) Journal of Veterinary Diagnostic Investigation. Vol 30 (6) 837-847. DOI:10.1177/1040638718800170

Robbins K, Dickey AM, Clawson ML, and Loy JD Application and evaluation of the matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry method to identify Moraxella bovoculi and Moraxella bovis isolates from cattle (2018). Journal of Veterinary Diagnostic Investigation. DOI: 10.1177/1040638718789725

Loy, JD and Clawson ML. Rapid typing of Mannheimia haemolytica major genotypes 1 and 2 using MALDI-TOF mass spectrometry. (2017) Journal of Microbiological Methods. Vol 136. 30-33. DOI: 10.1016/j.mimet2017.03.002

Workman AM, Kuehn LA, McDaneld TG, Clawson ML, Chitko-McKown CD, and Loy, JD. Evaluation of bovine coronavirus antibody levels, virus shedding, and respiratory disease incidence throughout the beef cattle production cycle. (2017). American Journal of Veterinary Research. Vol 78 (9) 1065-1076. DOI:10.2460/ajvr.78.9.1065

Dickey, A. Loy JD, Bono JL, Smith T.P.L., Apley M., Lubbers B., DeDonder KD, Capik SF, Larson RL, White BJ, and Clawson M. (2016). Distinct genomic differences between Moraxella bovoculi isolates acquired from the eyes of cattle with conjunctivitis versus the deep nasopharynx of asymptomatic cattle. Veterinary Research. 47 (31). DOI 10.1186/s13567-016-0316-2

Loy, JD and BW Brodersen. (2014). Moraxella spp. isolated from field outbreaks of Infectious Bovine Keratoconjunctivitis: a retrospective study of case submissions from 2010-2013. Journal of Veterinary Diagnostic Investigation. Vol 26:6. DOI: 10.1177/1040638714551403 

Presentations

Dr. Loy has delivered numerous continuing education seminars throughout the United States, primarily focused on bovine pinkeye or infectious bovine keratoconjunctivitis and diagnostics for bovine respiratory disease.

Awards and Honors

• Outstanding Young Alumni Award, Iowa State Alumni Association, 2021
• National Strategic Research Institute Faculty Fellow, 2021-Current
• AAVLD Award for Excellence in Diagnostic Veterinary Microbiology, 2019
• Bacteriology Committee Co-Chair American Association of Veterinary Laboratory Diagnosticians, 2019-2021
• Board of Governors, American College of Veterinary Microbiologists, 2017-2020
• Examination Committee Chair, American College of Veterinary Microbiologists, 2018-Current
• Junior Faculty for Excellence in Research Nominee, University of Nebraska-Lincoln, 2017
• Bovine Working Group, Clinical Laboratory Standards Institute, Subcommittee on Veterinary Antimicrobial Susceptibility Testing, 2015-Current